LOCUS       sequence-107246-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by 
            BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV11K
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(106..783)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cccagtagag
       61 accgcctggc tctagtagcg atctacacta gcactatcag cgttattaag caccggtgga
      121 gtgacgacct tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgggaggt
      181 gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt ttttagccat
      241 gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct ttcagtttca gacgcatttt
      301 gatttcacct ttcagagcac cgtcttccgg gtacatacgt tcggtggaag cttcccaacc
      361 catggttttt ttctgcataa ccggaccgtc ggacgggaag ttggtaccac gcagtttaac
      421 tttgtagatg aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa caacaccacc
      481 gtcttcgaag ttcataacac gttcccattt gaaaccttcc gggaaggaca gtttcaggta
      541 gtccgggatg tcagccgggt gtttaacgta agctttggaa ccgtactgga actgcgggga
      601 caggatgtcc caagcgaacg gcagcggacc acctttggta actttcagtt tagcggtctg
      661 ggtaccttcg tacggacgac cttcaccttc accttcgatt tcgaactcgt gaccgttaac
      721 ggaaccttcc atacgaactt tgaaacgcat gaactctttg ataacgtctt cggaggaagc
      781 catctagtat t
//
