LOCUS       sequence-107314-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    464..488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(488..507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(489..507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgaggg
       61 taccttagtt aaagtgtccg tctcggaaaa gcttctgggc aatgtgcaga gggtttccaa
      121 agcggaagtt ctccccactg aacaaggcta agatgagttc attctgctgc tcagcactgt
      181 cttcaggaaa ctggggcaga aactgctgga ggtgttcacg ttgagaatca cttaacactt
      241 cctgccatgt tgagaggctg acaacatcaa agaagatctc aggatcctcc agaaggtcct
      301 cgggcagact aactctggtg cctcccagga ggcaatcctc catgatgcgc gtgccatggc
      361 catctccaca cggaccaagt tccagaggat ctgtcagcat atggtctaag gaatccatat
      421 tatgtatatc tccttcttat acttaactaa tatactaaga tggggaattg ttatccgctc
      481 acaattcccc tatagtgagt cgtattaatt tcgattatgc ggccgtgtac aatacgatta
      541 ctttctgttc gacttaagca ttatgcggcc gccactgtgc tggatatctg cagaattcca
      601 ccacactgga ctagtggatc cgagctcggt acctcatttg gtttcctgag ctttctttgc
      661 gttctgtttt tcttttgcct tttctactag tggtattaac tgctggtgtt ctgctaaagt
      721 ctttaacaat tccataatga aaggcagata attatgcttc cttctgatat tctcaatctt
      781 gtatcttttt aatttctgta cttcctcttc aataagcatc tgatttttgg caacttctga
      841 ctgaatagca cttaacatac tattaccttg atctgtatcc atgg
//
