LOCUS       sequence-107321-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myristoylated-FLAG-tagged Akt2, constitutively active.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-FLAG-Akt2-CA
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..145
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             164..187
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
       61 ttttgttaga cgaagcttgg gctgcaggtc gactctagag gatcgaattc ttcgaagcta
      121 ggccatgggt tcctccaaat ccaagcccaa ggctagcgcc atggactaca aggacgatga
      181 cgataaggga attgatccaa tgaatgaggt gtctgtcatc aaagaaggct ggctccacaa
      241 gcgtggtgaa tacatcaaga cctggaggcc acggtacttc ctgctgaaga gcgacggctc
      301 cttcattggg tacaaggaga ggcccgaggc ccctgatcag actctacccc ccttaaacaa
      361 cttctccgta gcagaatgcc agctgatgaa gaccgagagg ccgcgaccca acacctttgt
      421 catacgctgc ctgcagtgga ccacagtcat cgagaggacc ttccacgtgg attctccaga
      481 cgagagggag gagtggatgc gggccatcca gatggtcgcc aacagcctca agcagcgggc
      541 cccaggcgag gaccccatgg accacaagtg tggctccccc agtgactcct ccacgactga
      601 ggagatggaa gtggcggtca gcaaggcacg ggctaaagtg accatgaatg acttcgacta
      661 tctcaaactc cttggcaagg gaacctttgg caaagtcatc ctggtgcggg agaaggccac
      721 tggccgctac tacgccatga agatcctgcg gaaggaagtc atcattgcca aggatgaagt
      781 cgctcacaca gtcaccgaga gccgggtcct ccagaacacc aggcacccgt tcctcactgc
      841 gctgaagtat gccttccaga cccacgaccg cctgtgcttt gtgatggagt atgccaacgg
      901 gggtgagctg ttcttccacc tgtcccggga gcgcgtcttc acagaggagc gggcccggtt
      961 ttatggtgca gagattgtct cggctcttga gtacttgcac tcgcgggacg tggtataccg
     1021 cgacatcaag ctggaaaa
//
