LOCUS       sequence-107380-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme 
            design for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC625
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(402..599)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgcacttcat gatgcatgtt tgttccttat tggttagctt tgatgttgtg aagtcattga
       61 cacagtctgt gaaacatctt tctaccagat tagagtacaa acgcatgaaa tccttcattt
      121 gcttttgttc cactactttt tggaactctt gttgttcttt ggagttcaat gcgtccatct
      181 ttacagtcct gtcttattgt tcttgatttg tgccccgtaa aatactgtta cttggttctg
      241 gcgaggtatt ggatagttcc tttttataaa ggccatgaag ctttttcttt ccaatttttt
      301 ttttttcgtc attatagaaa tcattacgac cgagattccc gggtcgatga attcgagctc
      361 gttttcgaca ctggatggcg gcgttagtat cgaatcgaca gcagtatagc gaccagcatt
      421 cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg
      481 atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca
      541 atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta
      601 ctgattataa ttggccagtc tttttcaaat aagcattcca accagca
//
