LOCUS       sequence-107458-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN array receiving vector with half-site NI RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, 
            May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS
  TITLE     Genome engineering of isogenic human ES cells to model autism 
            disorders.
  JOURNAL   Nucleic Acids Res. 2015 Mar 11. pii: gkv164.
  PUBMED    25765640
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Mar 11. pii: gkv164."
COMMENT     Sequence Label: AI-CN441
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(74..661)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt
       61 cgaagggccc ttaaaagttt atctcgccgt tattaaattt ccgtctgact tcctctaagg
      121 ttaatgtgcc ggctttaatc atttctccac caattaaaag ctcttctaca ctaagaacag
      181 ctccattaca attagtgata tgatttaatc gtgtaagctg agctttgtag tttcctttaa
      241 agtgaccact cacaaataaa aacttaaatt ccgttacaga agatggatag actttccacc
      301 attcattagg gttgatatgt ttgtttcgtg tttgattttc ttcgacatat cgttgcattt
      361 catctgcttg gccaattggc agattataac ctccgctata agctttagta tccacgatca
      421 caccgtaatc aataggagat ccgacagtat aaattgctcc gtccggtttc cttgatccac
      481 ccaaatgttt acctctatat ccataaactt tcataaaaaa ttccattacc ttcatttcaa
      541 gaattctatc ctgagtggaa tttctggcaa tttcaattaa ttcaatatat tcatgaggca
      601 catatttcaa tttatgacga agttcagatt tcttctcctc cagttcactt ttgactagtt
      661 gggatcccgc gactcgatgg gaagttctct cgggaatccg ccggttggtt cttttgatca
      721 atgcgggagc atgaggcaga ccctttttga ctgcatcgag cgcgggtcgt ccaccaagac
      781 atgccaacgc caccagatgg tcattcgtta acgcagccaa cgcgggatcg ggcctggaca
      841 attgggctac gatggactcc
//
