LOCUS       sequence-107508-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice 
            ubiquitin promoter for transient expression in protoplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Xie K, Minkenberg B, Yang Y
  TITLE     Boosting CRISPR/Cas9 multiplex editing capability with the 
            endogenous tRNA-processing system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 
            10.1073/pnas.1420294112. Epub 2015 Mar 2.
  PUBMED    25733849
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420294112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3570-5"
COMMENT     Sequence Label: pRGE32
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
       61 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
      121 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
      181 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
      241 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
      301 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
      361 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
      421 aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
      481 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
      541 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
      601 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
      661 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
      721 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
      781 cagagcaaga acggctacgc cggctacatt gacggcgg
//
