LOCUS       sequence-107557-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 
            sgRNAs into pDe-CAS9 or pCAS9-TPC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Schiml S, Fauser F, Puchta H
  TITLE     The CRISPR/Cas system can be used as nuclease for in planta gene 
            targeting and as paired nickases for directed mutagenesis in 
            Arabidopsis resulting in heritable progeny.
  JOURNAL   Plant J. 2014 Oct 18. doi: 10.1111/tpj.12704.
  PUBMED    25327456
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 18. doi: 10.1111/tpj.12704."
COMMENT     Sequence Label: pEn-C1.1
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..28
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10..27
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    109..208
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    227..251
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(263..338)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag
       61 ctctcccata tggtcgacct gcaggcggcc gcgaattcac tagtgattca aataatgatt
      121 ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatt attttttata
      181 atgccaactt tgtacaagaa agctgggtcc tcaggacgcg ttaatgccaa ctttgtacaa
      241 gaaagctggg tctagaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
      301 ctagccttat tttaacttgc tatttctagc tctaaaacag gtcttctcga agaccccaat
      361 cactacttcg actctagctg tatataaact cagcttcgtt ttcttatcta agcgatgtgg
      421 gacttttgaa gattgttttc aacttaaatg ggcctatata agaaatacta ttgttctttc
      481 ccatataaat gggcctgctt ctcttctttc agattcccag gggccttttg aagattatct
      541 tcatatctta agaatgaaga tgttttattc aatcaaattc ttgaaggttc gatgcctaat
      601 cattctaatc ctgggacaaa ctatgaaaca agatacaaaa actccgaatg gaaagttaaa
      661 aagaa
//
