LOCUS       sequence-107582-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary expression vector with A.th. codon-optimized Cas9 and Gateway
            destination sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Fauser F, Schiml S, Puchta H
  TITLE     Both CRISPR/Cas-based nucleases and nickases can be used efficiently
            for genome engineering in Arabidopsis thaliana.
  JOURNAL   Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 
            Jun 17.
  PUBMED    24836556
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12554";
            journalName: "Plant J"; date: "2014-07"; volume: "79"; issue: "2"; 
            pages: "348-59"
COMMENT     Sequence Label: pDe-CAS9
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(77..83,84..101,102..107))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(768..788)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgacggatcc
       61 taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      121 aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 ttttgtacaa acttgttagg gataacaggc ctagggagct caagcctata ctgtacttaa
      301 cttgattgca taattacttg atcatagact catagtaaac ttgattacac agataagtga
      361 agaaacaaac caattcaaga cataaccaaa gagaggtgaa agactgtttt atatgtctaa
      421 cattgcacct taatatcaca ctgttagttc ctttcttact taaattcaac ccattaaagt
      481 aaaaacaaca gataataata atttgagaat gaacaaaagg accatatcat ttattaactc
      541 ttatccatcc atttgcattt tgatgtccga aaacaaaaac tgaaagaaca cagtaaatta
      601 caagcagaac aaatgataga agaaaacagc ttttccaatg ccataatact caaacttagt
      661 aggattctgg tgtgtgggca atgaaactga tgcattgaac ttgacgaacg ttgtcgaaac
      721 cgatgatacg gacgaaagct gggaggcctg gagctcggcg cgcctcaaac cttcctcttc
      781 ttcttaggat cagcccttga atcaccaccg agctgtgaga gatcgatcct agtctcgtag
      841 agtccagtga tagactgat
//
