LOCUS       sequence-107604-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the adaptor protein AP180 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Norris FA, Ungewickell E, Majerus PW
  TITLE     Inositol hexakisphosphate binds to clathrin assembly protein 3 
            (AP-3/AP180) and inhibits clathrin cage assembly in vitro.
  JOURNAL   J Biol Chem. 1995 Jan 6;270(1):214-7.
  PUBMED    7814377
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1995-01-6"; volume: "270(1)"; pages: "214-7"
COMMENT     Sequence Label: H6-AP180
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(3..17,18..23,24..40,41..47)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    23..39
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    55..71
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatcataaaa aatttatttg ctttgtgagc ggataacaat tataatagat tcaattgtga
       61 gcggataaca atttcacaca gaattcatta aagaggagaa attaactatg agaggatctc
      121 accatcacca tcaccatacg gatccgcatg cgagctcggt acccccggtg aagatgtcgg
      181 gccaaacgct cacggatcgg atcgctgccg ctcagtacag cgtgactggc tctgctgtag
      241 caagagccgt ctgcaaagcc accactcatg aggtgatggg ccccaagaag aagcacctgg
      301 actatttgat ccaggctacc aacgagacca atgtcaatat tcctcagatg gcggatactc
      361 tctttgagcg ggcgacaaac agcagctggg tggtggtatt taaagcttta gtgaccacac
      421 accacctcat ggtgcacgga aatgagagat ttattcagta tttggcctct agaaatacgc
      481 tgttcaacct gagcaacttt ctagataaga gtggatccca cggttatgat atgtccacgt
      541 tcatacgacg ttatagtaga tacttgaatg agaaggcttt ctcctacaga cagatggcat
      601 tcgactttgc cagagtgaag aaaggggccg acggtgtaat gaggacaatg gttcctgaaa
      661 agttgctgaa gagcatgcca atcctgcagg ggcagatcga cgcgctgctg gagttcgatg
      721 tgcatccaaa tgagctaaca aatggtgtca taaatgctgc atttatgctt cttttcaaag
      781 atctcatcaa actgtttgct tgctacaatg acggcgtcat taacttactt gaaaaatttt
      841 ttgagatgaa gaaaggtcaa tgcaaagacg cactagaaat ttacaaacga tttctaacta
      901 gaatgactcg agtctccgaa ttcctcaagg tcgcggagca agttggtatt gataaaggtg
      961 acattcccga cctcacacag gctcccagca gtcttatgga gacccttgaa caacatctaa
     1021 ataccttaga aggaaagaaa cctggaa
//
