LOCUS       sequence-107640-         363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with BiTet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_BiTet
FEATURES             Location/Qualifiers
     source          1..363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(24..48)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    join(75..93,94..116,117..135,136..158,159..177,178..200,
                     201..219,220..242,243..261,262..284,285..303,304..326,
                     327..345)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
       61 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      121 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      181 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      241 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      301 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      361 ctc
//
