LOCUS       sequence-107658-         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  adenoviral shuttle vector containing N-terminal Flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Lingfang Zeng, Qingbo Xu
  TITLE     pShuttle-Flag-HA vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-Flag-HA
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..48)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(49..75)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(148..171)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(193..212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(194..212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(257..460)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(286..306)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctagtcactt acttacttaa gcgcgtagtc tggcacgtcg taggggtatg cataatcagg
       61 aacatcatac ggataatcca gggatcctct agagtcgacc tgcaggcatg caagcttatc
      121 gatacgcgtg gtaccagatc tgaattcctt gtcatcgtcg tccttgtagt ccatgctagc
      181 cagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt
      241 ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      301 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
      361 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
      421 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
      481 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
      541 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
      601 gatgtactgc caagtgggca gtttaccgt
//
