LOCUS       sequence-107663-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pUC19 vector contains a tetramethylrosamine (TMR) binding 
            riboswitch, controlling the translation initaition rate of 
            luciferase gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-TMR10pUC19
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             87..95
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(166..184)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttaatcatcg gctcgtataa tgtgtgggat tcccgtaccg gatcgcgact ggcgagagcc
       61 aggtaacgaa tcgatccaag attcgttaag gaggtccata tggaagacgc caaaaacata
      121 aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat
      181 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      241 gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
      301 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
      361 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
      421 atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt
      481 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
      541 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
      601 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat
      661 cgtgac
//
