LOCUS       sequence-107784-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP under the UbC promoter. This promoter expresses 
            transgenes in neurons at higher levels than plasmids with the CMV. 
            Optimal for tracing axons in tissue clearing procedures. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, 
            Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, 
            Lemmon VP, Lee JK, Tsoulfas P
  TITLE     3D Imaging of Axons in Transparent Spinal Cords from Rodents and 
            Nonhuman Primates
  JOURNAL   Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.
  PUBMED    26023683
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eneuro. 
            2015 Mar-Apr"; volume: "2(2). doi"; pages: " 
            10.1523/ENEURO.0001-15.2015"
COMMENT     Sequence Label: pAAV-UbC-eGFP
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(188..901,902..904,905..907))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(191..901,902..904,905..907))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(233..254)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     582..601
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     841..862
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(935..954)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gtcttcccaa cttgcccctt gctccatacc acccccctcc accccataat attatagaag
       61 gacacctagt cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact
      121 ggagtggcaa cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg
      181 tagatcttca cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      241 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagagcg gactgggtgc
      301 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      361 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      421 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      481 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      541 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      601 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      661 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
      721 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      781 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      841 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
      901 tcaccatggt ggatccttaa gcttgatgag acagcacaat aaccagcacg ttgcccagga
      961 gctgtaggaa aa
//
