LOCUS       sequence-107792-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.HO5.loxP
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(168..201)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
       61 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
      121 tgatagtgct agtgtagatc gctactagag ccaggcggtc tctactgata acttcgtata
      181 gcatacatta tacgaagtta ttgctagcta gtttcatgat tttctgttac acctaacttt
      241 ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc
      301 acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc
      361 ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca
      421 ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat
      481 cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca
      541 ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa
      601 acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc
      661 aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct
      721 ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag
      781 gccaaggacg cagatggc
//
