LOCUS       sequence-107849-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle vector compatible with Flp-mediated (Flp-in) transgene 
            integration that allows for conditional Luciferase expression upon 
            integration. The vector contains an FRT site and two mutant loxP 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pFrt-invCAG-Luc
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     215..232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    357..478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    603..737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
       61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      241 agacctgcag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      301 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      361 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      421 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      481 atgtctggat gctagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      541 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      601 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
      661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      721 cttatcatgt ctggatctag cgatcataat cagccatacc acatttgtag aggttttact
      781 tgctttaaaa aacctcccac acctccccct gaacctgaaa cata
//
