LOCUS       sequence-107890-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Shen WF, Krishnan K, Lawrence HJ, Largman C
  TITLE     The HOX homeodomain proteins block CBP histone acetyltransferase 
            activity
  JOURNAL   Mol Cell Biol. 2001 Nov;21(21):7509-22
  PUBMED    11585930
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2001-11"; volume: "21(21)"; pages: "7509-2"
COMMENT     Sequence Label: CBP-HAT-pET
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             82..114
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggctagc atgactggtg gacagcaaat gggtcgcgga
      121 tccacgtccc gtgtatataa attttgcagt aaacttgcag aggtctttga acaagaaatt
      181 gaccctgtca tgcagtctct tggatattgc tgtggacgaa agtatgagtt ctccccacag
      241 actttgtgct gttacggaaa gcagctgtgt acaattcctc gtgatgcagc ctactacagc
      301 tatcagaata ggtatcattt ctgtgagaag tgtttcacag agatccaggg cgagaatgtg
      361 accctgggtg acgacccttc ccaacctcag acgacaattt ccaaggatca atttgaaaag
      421 aagaaaaatg ataccttaga tcctgaacct tttgttgact gcaaagagtg tggccggaag
      481 atgcatcaga tttgtgttct acactatgac atcatttggc cttcaggttt tgtgtgtgac
      541 aactgtttga agaaaactgg cagacctcgg aaagaaaaca aattcagtgc taagaggctg
      601 cagaccac
//
