LOCUS       sequence-108017-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLg
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     276..297
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(343..393)
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     protein_bind    complement(396..420)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcttacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
       61 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      121 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      181 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      241 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      301 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atttcttcct ttgagatgct
      361 ttcgaatttc ttgatcaaat ctgcgacacc catgaagcct gcttttttgt acaaacttgc
      421 ttgatcaaaa tttgattttg gaaaatattt ctctctaaat gtagaaatta actttaaaaa
      481 aatgagtgtc aaaattttat ttcctagaat aaaatctcaa tcaaaggaat agcaagttag
      541 caaatgtaat tgttttgatt tgatttgatg aattgtgcat atcaacttag gctatatata
      601 gttgtcatgt gtgggtgtat tgattttatc gtcacaacaa taatagaata tttgtttgct
      661 attataaacg gctaccggat ttggtcggct ttttactaag acgaggttga atttccacaa
      721 cttattattt ttttttttac ctttttggaa tacacttata ttattttata ttaatgtaaa
      781 gaaactttct atcacttgaa caaaaagaaa gttttctttt gtttttattt aaccacgtgg
      841 acatacttgt ttttatttgg cagtaaaaca agtatgcaat a
//
