LOCUS       sequence-108018-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.
            A retroviral expression vector with the puromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFN-Tel-TAL/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      183..192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..233
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             246..266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggggtggacc atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt
       61 ggtacgggaa ttcgtttagt gaaccgtcag aattaaccat ggactacaaa gaccatgacg
      121 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagctt gcggccggcc
      181 gcgccaccat gggaaaacct attcctaatc ctctgctggg cctggattct accggaggca
      241 tggcccctaa gaaaaagcgg aaggtggacg gcggagtgga cctgagaaca ctgggatatt
      301 ctcagcagca gcaggagaag atcaagccca aggtgagatc cacagtggcc cagcaccacg
      361 aagccctggt gggacacgga tttacacacg cccacattgt ggccctgtct cagcaccctg
      421 ccgccctggg aacagtggcc gtgaaatatc aggatatgat tgccgccctg cctgaggcca
      481 cacacgaagc cattgtggga gtgggaaaac agtggtctgg agccagagcc ctggaagccc
      541 tgctgacagt ggccggagaa ctgagaggac ctcctctgca gctggataca ggacagctgc
      601 tgaagattgc caaaaggggc ggagtgaccg cggtggaagc cgtgcacgcc tggagaaatg
      661 ccctgacagg agcccctctg aacctgaccc ccgaacaggt ggtggccatt gccagcaaca
      721 tcggcggcaa gcaggccctg gaaaccgtgc agagactgct gcccgtgctg tgccaggccc
      781 atggcctgac acctgaacag gtggtggcta tcgcctctaa caacggagga aaacaggctc
      841 tggaaacagt gca
//
