LOCUS       sequence-108028-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS, Ho1, and PcyA constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500273n";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "820-31"
COMMENT     Sequence Label: pSR43.6r
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(139..166)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(182..253)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
       61 ccagctggca attccggttc gcttgctaag cttgcatgcc tgcaggtcga ctctagagga
      121 tccccgggta cctataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt
      181 agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
      241 ttcgttttat ttgatgcctg gtctagatta ttggataaca tcaaataaga cttggctcat
      301 ataccgttcc gcccaagctt ccccaaaagc tttttccagt acccgacggg ttttatcatt
      361 tttctgttgt tgttggcagt aatgaatttg cccctgacgg tgctccaaag tttgagcttc
      421 agacaagggt tcggcaacga tggattggtg acaatgaatt tgcaaaaagt ccactaccct
      481 ttgtacaaat ctttcttctt cagtgacatt gctgggacgg atgaataaac aatattcaga
      541 aaatatttct ccccaggggg gcaattcccg ttgttgctca aattctggct ggcctagctc
      601 tgccaatgat ttttggtacg ctgcgggcaa ttggcgatcg ctttgggtgg gggatagatc
      661 cgcaatagcc gcacttactc caccggggcc ggccacaatg tcacagccaa acaaaggtag
      721 accgtataaa ggctcaggaa acattacaca gtggagaata tccaaccctt tgcccacctt
      781 ggccaactcc aaatgcattt tgcgaaactg gggcgtttgg tagcaccgat tttcaatcac
      841 taacttttcc ccttccaggc ggccttctac gtagcccaaa tcctccggca attgataggg
      901 cttgaggggt aaactttgcc aactagcggc gatcgccagg gccaactgtt gaatcatcgg
      961 gttcaac
//
