LOCUS       sequence-108054-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             148..165
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             208..231
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat ataccatggg cagcagccat caccatcatc accacagcca ggatccgaat
      181 tcgagctcgg cgcgcctgca ggtcgaggac tacaaggacg atgatgacaa gatgacgggc
      241 aacgccgggg agtggtgcct catggaaagc gaccccgggg tcttcaccga gctcattaaa
      301 ggattcggtt gccgaggagc ccaagtagaa gaaatatgga gtttagagcc tgagaatttt
      361 gaaaaattaa agccagttca tgggttaatt tttcttttca agtggcagcc aggagaagaa
      421 ccagcaggct ctgtggttca ggactcccga cttgacacga tattttttgc taagcaggta
      481 attaataatg cttgtgctac tcaagccata gtgagtgtgt tactgaactg tacccaccag
      541 gatgtccatt taggcgagac attatcagag tttaaagaat tttcacaaag ttttgatgca
      601 gctatgaaag gcttggcact gagcaattca gatgtgattc gacaagtaca caacagtttc
      661 gccagacagc aaatgtttga atttgatacg aagacatcag caaaagaaga agatgctttt
      721 cactttgtca gttatgttcc tgttaatggg agactgtatg aattagatgg attaagagaa
      781 ggaccgattg atttaggtgc atgcaatcaa gatgattgga tcagtgcagt aaggcctgtc
      841 atagaa
//
