LOCUS       sequence-108058-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    456..480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(480..499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(481..499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgctggcgtt caaatttcgc agcagcggtt tctttaccag actcgagggt accttaatca
       61 ctccgggaag caagaatttg cttcagcagc cgatggaaat actgctgctg ggagttgagg
      121 tagcgcttgt actgtgactt gaagcataac tgccggtact tgaccacctc ggggttaaag
      181 tgtccgtctc ggaaaagctt ctgggcaatg tgcagagggt ttccaaagcg gaagttctcc
      241 ccactgaaca aggctaagat gagttcattc tgctgctcag cactgtcttc aggaaactgg
      301 ggcagaaact gctggaggtg ttcacgttga gaatcactta acacttcctg ccatgttgag
      361 aggctgacaa catcaaagaa gatctcagga tcctccagaa ggtcctcggg catatgtata
      421 tctccttctt atacttaact aatatactaa gatggggaat tgttatccgc tcacaattcc
      481 cctatagtga gtcgtattaa tttcgattat gcggccgtgt acaatacgat tactttctgt
      541 tcgacttaag cattatgggc cgccactgtg ctggatatct gcagaattcc accacactgg
      601 actagtggat ccgagctcgg tacctcattt ggtttcctga gctttctttg cgttctgttt
      661 ttcttttgcc ttttctacta gtggtattaa ctgctggtgt tctgctaaag tctttaacaa
      721 ttccataatg aaaggcagat aattatgctt ccttctgata ttctcaatct tgtatctttt
      781 taatttctgt acttcctctt caataagcat ctgatttttg gcaacttctg actgaatagc
      841 acttaacata ctattacctt g
//
