LOCUS       sequence-108137-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational Luciferase Reporter encoding a mutated fragment of the
            SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage 
            KM, Salkeni MA, Ruppert JM
  TITLE     MicroRNAs 206 and 21 cooperate to promote RAS-extracellular 
            signal-regulated kinase signaling by suppressing the translation of 
            RASA1 and SPRED1.
  JOURNAL   Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 
            10.1128/MCB.00480-14. Epub 2014 Sep 8.
  PUBMED    25202123
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00480-14"; journalName: "Mol Cell Biol"; date: 
            "2014-11-15- 15"; volume: "34"; issue: "22"; pages: "4143-64"
COMMENT     Sequence Label: pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaaataa aagatccttt attaagctta actgtttaaa cctatgatat cacgcacgcg
       61 ttgaaaaacc tgtaaataag cacatgagac tacatttcat aattaatggt aaagatacag
      121 ttactccttg atttttcaaa taaactataa tatagtaatc caacttattc tagtgttgag
      181 gtacactata attttttatt actgtcctaa taacctttcc tttagattat tcgttatttt
      241 tggttatatg gcgagttaag taaaacaatg aaactgactt gaatataaaa aaatcagtag
      301 tcctgggtta aacaattgcc acttaaggtc attagcttag ctgcaacatg ttaccaaacc
      361 aataaatata acattattga tctagagact taaaaaaaaa tctgaataca attaaggatg
      421 aataaatatg ttattaagat gaactatatg tgaactttcg gtttaaaagt atacttttaa
      481 agaacagagc taattatcaa aagcttccat ttttactttc tcctccagta attatctctt
      541 atgtgataat ttgataagct ctaggagaat aagaaaaagt ccttttataa ttcatgacag
      601 gtttaatcag gtaattccag gagggaaaaa aaccattaaa acaaattctt atgataatca
      661 tctgctatgt ccaattacac gggtatgaag taacagaaat ggttacaaac ttattatgta
      721 ttatttacat tatatactca aaactatcaa gctagttaac tatatctgag gagacacatt
      781 atgacatgag ttaaacagtt tttcaacgcg tctatgccgg cctatgagct ccctcactag
      841 tgcgcagctt tcatcactcg agcaatttgg actttccgcc c
//
