LOCUS       sequence-108143-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translational Luciferase Reporter encoding a mutated fragment of the
            SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was 
            mutated..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage 
            KM, Salkeni MA, Ruppert JM
  TITLE     MicroRNAs 206 and 21 cooperate to promote RAS-extracellular 
            signal-regulated kinase signaling by suppressing the translation of 
            RASA1 and SPRED1.
  JOURNAL   Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 
            10.1128/MCB.00480-14. Epub 2014 Sep 8.
  PUBMED    25202123
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00480-14"; journalName: "Mol Cell Biol"; date: 
            "2014-11-15- 15"; volume: "34"; issue: "22"; pages: "4143-64"
COMMENT     Sequence Label: pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agatccaatg aaaataaaag atcctttatt aagcttaact gtttaaacct atgacatcac
       61 gcacgcgttg aaaaacctgt aaataagcac atgagactac atttcataat taatggtaaa
      121 gatacagtta ctccttgatt tttcaaataa actataatat agtaatccaa cttattctag
      181 tgttgaggta cactataatt ttttattact gtcctaataa cctttccttt agattattcg
      241 ttatttttgg ttatatggcg agttaagtaa aacaatgaaa ctgacttgaa tataaaaaaa
      301 tcagtagtcc tgggttaaac aattgccact taaggtcatt gatctagctg caacatgtta
      361 ccaaaccaat aaatataaca ttattagctt agagacttaa aaaaaaatct gaatacaatt
      421 aaggatgaat aaatatgtta ttaagatgaa ctatatgtga actttcggtt taaaagtata
      481 cttttaaaga acagagctaa ttatcaaaag cttccatttt tactttctcc tccagtaatt
      541 atctcttatg tgataatttg ataagctcta ggagaataag aaaaagtcct tttataattc
      601 atgacaggtt taatcaggta attccaggag ggaaaaaaac cattaaaaca aattcttatg
      661 ataatcatct gctatgtcca attacacggg tatgaagtaa cagaaatggt tacaaactta
      721 ttatgtatta tttacattat atactcaaaa ctatcaagct agttaactat atctgaggag
      781 acacattatg acatgagtt
//
