LOCUS       sequence-108409-        5652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  His3 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRH3
FEATURES             Location/Qualifiers
     source          1..5652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..124
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        271..457
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             458..1120
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1380..1835)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1517..1538)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1729..1748
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(1812..2192)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     1961..1983
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1975..1992
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1976..1992
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2002..2021
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2002..2020
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2029..2136
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     2045..2061
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2046..2062
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2149..2169)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2149..2167)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2188..2204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2188..2204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2201..2223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2212..2228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2236..2242,2243..2260,2261..2266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2281..2302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2419..2436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2590..3178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2670..2689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3349..4140,4141..4209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3972..3991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4210..4301)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4336..4369
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4944..4977
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
       61 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
      121 tgtactgaga gtgcaccata aattcccgtt ttaagagctt ggtgagcgct aggagtcact
      181 gccaggtatc gtttgaacac ggcattagtc agggaagtca taacacagtc ctttcccgca
      241 attttctttt tctattactc ttggcctcct ctagtacact ctatattttt ttatgcctcg
      301 gtaatgattt tcattttttt ttttccccta gcggatgact cttttttttt cttagcgatt
      361 ggcattatca cataatgaat tatacattat ataaagtaat gtgatttctt cgaagaatat
      421 actaaaaaat gagcaggcaa gataaacgaa ggcaaagatg acagagcaga aagccctagt
      481 aaagcgtatt acaaatgaaa ccaagattca gattgcgatc tctttaaagg gtggtcccct
      541 agcgatagag cactcgatct tcccagaaaa agaggcagaa gcagtagcag aacaggccac
      601 acaatcgcaa gtgattaacg tccacacagg tatagggttt ctggaccata tgatacatgc
      661 tctggccaag cattccggct ggtcgctaat cgttgagtgc attggtgact tacacataga
      721 cgaccatcac accactgaag actgcgggat tgctctcggt caagctttta aagaggccct
      781 aggggccgtg cgtggagtaa aaaggtttgg atcaggattt gcgcctttgg atgaggcact
      841 ttccagagcg gtggtagatc tttcgaacag gccgtacgca gttgtcgaac ttggtttgca
      901 aagggagaaa gtaggagatc tctcttgcga gatgatcccg cattttcttg aaagctttgc
      961 agaggctagc agaattaccc tccacgttga ttgtctgcga ggcaagaatg atcatcaccg
     1021 tagtgagagt gcgttcaagg ctcttgcggt tgccataaga gaagccacct cgcccaatgg
     1081 taccaacgat gttccctcca ccaaaggtgt tcttatgtag tgacaccgat tatttaaagc
     1141 tgcagcatac gatatatata catgtgtata tatgtatacc tatgaatgtc agtaagtatg
     1201 tatacgaaca gtatgatact gaagatgaca aggtaatgca tcattctata cgtgtcattc
     1261 tgaacgaggc gcgctttcct tttttctttt tgctttttct ttttttttct cttgaactcg
     1321 acggatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     1381 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     1441 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     1501 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     1561 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     1621 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     1681 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     1741 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1801 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     1861 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     1921 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     1981 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc
     2041 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
     2101 actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
     2161 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2341 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3001 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc agctgaagct
     4321 tcgacaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtctagag
     4381 atccccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtactataaa
     4441 aataattata atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa
     4501 agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg
     4561 ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt
     4621 tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc
     4681 gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag
     4741 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact
     4801 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca
     4861 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg
     4921 caagcgatgc gagaaccctt aatataactt cgtataatgt atgctatacg aagttattag
     4981 gtgatatcag atccactagc tataaggttg atattctcac gagtaagcgg cacttgctac
     5041 ttattgacat tgcagatttt tggctacaga aatagtatat tagagattat aattgctaat
     5101 caaatcaaaa tataaaatta gtaaaccaaa ccatttatac ccttccttag tagttatgga
     5161 ttgtttttta atgatatttc tgcaaaccaa agaaagattg ttatccagat agaatttagt
     5221 tttgatattc atttttttgt tgaagattga acgccatatc tgggcctcat aattcaaaag
     5281 acggtgccat tatcggtagc gtttcgcatt gtactggatg gcgcgccttc agaaatttca
     5341 cagttgatga atcgaaaaga atggtctcat tgcaacacgt aaggttaaga tgtccctttt
     5401 taccattata ggcaataaat gaatcataaa acgaccgtat actggtgaaa tagtagggag
     5461 aacgagtacc tgtagtaaaa agtataaatc atagttaatc gggcaatgtc cctcgatcaa
     5521 ggagtattgt gtcatgttcg agacaaacgc caacattttt gtttcttttg gacaaatgtt
     5581 gtttgcattt atgatccgtt atattttgat ctaatgtaga gttgcacgta gttcttactg
     5641 gcaaagaaat ct
//
