LOCUS       sequence-108428-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lys2 marker pXR vector uses for both plasmid assembly in vivo, as 
            well as targeted genomic integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Chou CC, Patel MT, Gartenberg MR
  TITLE     A series of conditional shuttle vectors for targeted genomic 
            integration in budding yeast.
  JOURNAL   FEMS Yeast Res. 2015 May;15(3):fov010. doi: 10.1093/femsyr/fov010. 
            Epub 2015 Mar 2.
  PUBMED    25736914
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2015-05"; volume: "15(3):fov010. doi"; pages: " 
            10.1093/femsyr/fov010. Epub 2015 Mar 2"
COMMENT     Sequence Label: pXRLy2
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..61
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    636..669
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcagctgaag cttcgacaac ccttaatata acttcgtata atgtatgcta tacgaagtta
       61 ttaggtctag agatccccgc gcacatttcc ccgaaaagtg ccacctgggt ccttttcatc
      121 acgtactata aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata
      181 gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact
      241 ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct caggtattaa
      301 tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac
      361 attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat
      421 atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttctt
      481 cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac aaaacataaa
      541 aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata cgaggcgcgt
      601 gtaagttaca ggcaagcgat gcgagaaccc ttaatataac ttcgtataat gtatgctata
      661 cgaagttatt aggtgatatc agatccacta gctataaggt tgatattctc acgagtaagc
      721 ggcacttgct acttattgac attgcagatt tttggctaca gaaatagtat attagagatt
      781 ataattgcta atcaaatcaa aatataaaat tagtaaacca aaccatttat acccttcctt
      841 agtagttatg gattgt
//
