LOCUS       sequence-108446-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses crtEBI genes for neurosporene production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: LacI_PlacO1_crtEBI_13
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             219..227
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
       61 caacgtaaat gcatgccgct tcgccttccg gccaccagaa tagcctgcga ttcaacgtcg
      121 acaattgtga gcggataaca attgacattg tgagcggata acaagatact gagcacatca
      181 gcaggacgca ctgaccgaat tcgattttta ggaacagtta aggaggttaa taatggcatt
      241 cgaacaacgt attgaagctg ctatggctgc tgctattgca cgtggccagg gtagcgaagc
      301 tccgtccaaa ctggcaactg cgctggacta cgctgtcacc cctggtggtg cgcgcattcg
      361 tccgactctg ctgctgtctg tcgccaccgc ttgcggcgat gaccgtcctg ccctgagcga
      421 cgcggctgcg gttgccctgg aactgatcca ttgcgcttct ctggtgcatg acgacctgcc
      481 gtgcttcgac gacgctgaaa tccgtcgtgg taagccaacc gttcatcgtg catacagcga
      541 accgctggct atcctgactg gtgattccct gatcgttatg ggtttcgaag ttctggcgcg
      601 tgcggcggct gatcagccac aacgtgccct gcaactggta acggctctgg cggtacgcac
      661 tggtatgccg atgggcatct gcgctggtca gggttgggaa agcgaatctc agatcaacct
      721 gtctgcatac caccgtgcc
//
