LOCUS       sequence-108468-        5084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with 
            CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.F3
FEATURES             Location/Qualifiers
     source          1..5084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(61..78)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(232..820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(312..331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1003..1097)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1118..1777)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1692..1711
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1939..1957
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1961..2004)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        2570..2756
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2757..3419
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             complement(3638..4315)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             4322..4333
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    4351..4367
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4375..4381,4382..4399,4400..4405))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4420..4441
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      join(5064..5084,1..37)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
       61 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
      241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      961 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     1021 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     1081 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     1141 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     1201 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     1261 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     1321 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     1381 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1441 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1501 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1561 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1621 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1681 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1741 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1801 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1861 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     1921 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     1981 ttagctttcg ctaaggatga tttctggaat tcgcggccgc gtatccttgc ttctttattt
     2041 ggcagcattt ttcaaaaata ataaaatgga agccgcgagt acgaacaatg atgtgttctg
     2101 ggaatacctc gtcaaaacaa gacaatggca aggattttct ttcatcaggc agaaagatct
     2161 ggatctgaat ggcatcattt tgtgatgtgt aaaagcggga ccttgttatt tcgacttttt
     2221 gcatcatgtt gatgcaattt gctacttttc cgacggtgcg ctccaacgga tgggtatttc
     2281 cttaataaca aggcatttct ctggaagttg gcttactgtt tgaaatcaca gccggtcaca
     2341 aaataaagta aaaaaactat ctctctccac aagaagtaat tacaggttgt atactacata
     2401 tgatcgtatt tctttatgaa cactaaggag tttcccgctg tgtaccgcaa tatccacttg
     2461 gtgagcgcta ggagtcactg ccaggtatcg tttgaacacg gcattagtca gggaagtcat
     2521 aacacagtcc tttcccgcaa ttttcttttt ctattactct tggcctcctc tagtacactc
     2581 tatatttttt tatgcctcgg taatgatttt catttttttt tttcccctag cggatgactc
     2641 tttttttttc ttagcgattg gcattatcac ataatgaatt atacattata taaagtaatg
     2701 tgatttcttc gaagaatata ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga
     2761 cagagcagaa agccctagta aagcgtatta caaatgaaac caagattcag attgcgatct
     2821 ctttaaaggg tggtccccta gcgatagagc actcgatctt cccagaaaaa gaggcagaag
     2881 cagtagcaga acaggccaca caatcgcaag tgattaacgt ccacacaggt atagggtttc
     2941 tggaccatat gatacatgct ctggccaagc attccggctg gtcgctaatc gttgagtgca
     3001 ttggtgactt acacatagac gaccatcaca ccactgaaga ctgcgggatt gctctcggtc
     3061 aagcttttaa agaggcccta ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg
     3121 cgcctttgga tgaggcactt tccagagcgg tggtagatct ttcgaacagg ccgtacgcag
     3181 ttgtcgaact tggtttgcaa agggagaaag taggagatct ctcttgcgag atgatcccgc
     3241 attttcttga aagctttgca gaggctagca gaattaccct ccacgttgat tgtctgcgag
     3301 gcaagaatga tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt gccataagag
     3361 aagccacctc gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagt
     3421 gacaccgatt atttaaagct gcagcatacg atatatatac atgtgtatat atgtatacct
     3481 atgaatgtca gtaagtatgt atacgaacag tatgatactg aagatgacaa ggtaatgcat
     3541 cattctatac gtgtcattct gaacgaggcg cgctttcctg gatccagtag agaccgcctg
     3601 gctctagtag cgatctacac tagcactatc agcgttatta agcaccggtg gagtgacgac
     3661 cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca
     3721 gtttgatgtc ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg
     3781 ttttaacttc agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt ttgatttcac
     3841 ctttcagagc accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt
     3901 ttttctgcat aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga
     3961 tgaactcacc gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga
     4021 agttcataac acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga
     4081 tgtcagccgg gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt
     4141 cccaagcgaa cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt
     4201 cgtacggacg accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt
     4261 ccatacgaac tttgaaacgc atgaactctt tgataacgtc ttcggaggaa gccatctagt
     4321 atttctcctc tttctctagt atgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4381 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4441 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4501 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggtctcatt ttgctcgaga
     4561 aacttctgtg gcttgacaga taaataactg cagtagtcgg tgcgtactaa ttgtttggtc
     4621 gtgtctgaaa aatcttgaat tttcagaaaa gaataagccc caaatgtcag tgatggtagt
     4681 agcagtactc ccctacgatt ttagatactt tagagagccc accttcagaa tcggaaggag
     4741 gataattttg taaagccctt ctgttttttc tcttgcataa cttatatttc cacatcaaaa
     4801 agtagtgtgc taagaaaaag gagacgagaa aaaggattac ggcactctct gcatctagac
     4861 atataccaaa agttgggttt gctcacgaaa ataccataat tgtggtgtca aaaaaatcct
     4921 gcctcataat accactgcag caattgtgga tgactaaaaa ataacttgca ttccacgatg
     4981 ttattttact ttataaagca cctgcaattt ttttttttgt attaactcat cgagtatgtc
     5041 tgatggatac gcggccgctg cagtccggca aaaaagggca aggt
//
