LOCUS       sequence-108479-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A member of an RBS library for mRFP1 with different translation 
            rates.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 185 to 219)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: mRFP1-19
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(185..219)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     promoter        272..374
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(441..460)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt
       61 cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga
      121 taacgtcttc agagctcgcc atgaatacac catgaagttg tgatcgtcga cgtcgtttct
      181 agaagctagc actgtaccta ggactgagct agccgtcaac ggatccccta gggaattccg
      241 cgctcgagga tatctggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
      301 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      361 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      421 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      481 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      541 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
      601 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      661 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacga
//
