LOCUS       sequence-108562-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible BiFC vector (pEarleyGate202-YC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGTQL1221YC
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    153..277
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(318..623)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRIVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(420..439)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
       61 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtg gttcatgact ttctgcttga
      121 ggtcgttagg gatcttacaa gcaggcctag gcaccacttt gtacaagaaa gctgaacgag
      181 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      241 cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt
      301 ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca
      361 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc
      421 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
      481 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcactat ccgtcgcccg
      541 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
      601 taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt
      661 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
      721 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
      781 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
      841 ttcatagcat acctctt
//
