LOCUS       sequence-108569-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-5
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             215..223
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggcgaaatca agatgcgctt aaagttaaaa gacggcggtc actacgacgc agaagttaaa
       61 acgacgtaca tggccaagaa accggttcag ttaccgggcg cttacaaaac cgacatcaaa
      121 ctcgacatca ccagccacaa cgaagactac accatcgtcg aacagtacga acgcgcagaa
      181 ggtcgtcaca gcaccggtgc tgaattcgta cacctaagga ggtgtacagc atgaaacgta
      241 aactcgagga acttttcacg ggggtcgtcc caattctggt agaattagac ggtgatgtaa
      301 acgggcacaa gtttagcgtc agcggagagg gcgaggggga cgccacctac ggtaaactta
      361 cccttaagtt tatttgcacg acgggcaaat tacccgttcc gtggccaacg ctcgtcacta
      421 ctttcggcta cggcgttcag tgcttcgcga gatatccgga tcacatgaag cagcacgatt
      481 tcttcaagtc ggccatgcca gagggttacg ttcaggagag aactatattt ttcaaggacg
      541 acggcaatta caagacacgc gccgaagtca agttcgaagg cgataccctg gttaaccgta
      601 tcgagttaaa aggtatcgat tttaaagaag acggaaacat tctcggtcac aaactcgaat
      661 acaactataa cagccataat gtctacatta tggctgac
//
