LOCUS       sequence-108584-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-8
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             246..254
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcgcatgtat ccggaggacg gcgctttaaa aggcgaaatc aagatgcgct taaagttaaa
       61 agacggcggt cactacgacg cagaagttaa aacgacgtac atggccaaga aaccggttca
      121 gttaccgggc gcttacaaaa ccgacatcaa actcgacatc accagccaca acgaagacta
      181 caccatcgtc gaacagtacg aacgcgcaga aggtcgtcac agcaccggtg ctgaattcgt
      241 acacctaagg aggtgtacag catgaaacgt aaactcgagg aacttttcac gggggtcgtc
      301 ccaattctgg tagaattaga cggtgatgta aacgggcaca agtttagcgt cagcggagag
      361 ggcgaggggg acgccaccta cggtaaactt acccttaagt ttatttgcac gacgggcaaa
      421 ttacccgttc cgtggccaac gctcgtcact actttcggct acggcgttca gtgcttcgcg
      481 agatatccgg atcacatgaa gcagcacgat ttcttcaagt cggccatgcc agagggttac
      541 gttcaggaga gaactatatt tttcaaggac gacggcaatt acaagacacg cgccgaagtc
      601 aagttcgaag gcgataccct ggttaaccgt atcgagttaa aaggtatcga ttttaaagaa
      661 gacggaaaca ttctcggtca caaactcgaa tacaactata acagccataa tgtctacatt
      721 atggctgaca aacagaagaa cggcattaag gttaacttc
//
