LOCUS       sequence-108585-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-8
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             160..168
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcctttggta actttgagtt tagcggtctg ggtaccttcg tacggacgac cttcgccttc
       61 cccttcgatt tcgaactcgt gaccgttaac cgagccctcc atgcgaactt taaaacgcat
      121 aaattctttg ataacgtctt cggagctcgc tttcatgata cctccttacg gaggtatttt
      181 ttgacgtcct tatagagttc gtccataccg agggtgatgc ctgccgccgt tacaaattcg
      241 agaaggacca tgtggtcgcg cttctcgttt ggatctttgg agagcgcgct ctgggtgctg
      301 agataatgat tgtccgggag gaggaccgga ccgtcgccga tcggcgtatt ctgttggtaa
      361 tggtcggcga gctgtaccga gccgtcctcg atgttatgac gaatcttaaa gtttgctttg
      421 ataccgttct tttgtttatc ggcggtaatg tagacgttat cggaaatcgc gttatactct
      481 aacttgtgac ctaaaatgtt gccgtcctct ttgaagtcga tacctttgag ttcgatgcga
      541 ttaacgagcg tatcgccctc gaacttaact tctgcgcggg ttttataatt accgtcgtcc
      601 ttgaagaaga tggtgcgctc ctgtacatag ccttcgggca tagccgattt gaagaaatcg
      661 tgttgcttca tgtggtctgg atagcgcgcg aagcactgga cgccccaggt aagggtggta
      721 actaacgt
//
