LOCUS       sequence-108666-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under 
            control of metallothionein promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  TITLE     pMT-IMD-V5-His
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMT-TAK1-V5-His
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..22)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(32..73)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcaatggtg atggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag
       61 ggataggctt accttcgaag ggccctctag actcgagcgg ccgccactgt gctggatatc
      121 tgcagaattc cgcattgtga tgcggtggga tgaccaccca gccttcttcc acggactcag
      181 agcctccgca caccgatccg ggcagcagtc cacagccctc atcctgcagg aaatgcatag
      241 gcggatgtga gtgtggatgc tgaagctgat ttggatgcgg atgaggatga ggttgcggat
      301 gatggccggc cgcaagttgg cgtgaagcgt actgctgctg cagattgttg taaagactct
      361 gaagaccctc cttgtccttc atcttgcgca gaagctcctg cttctgttcg cgctcggttc
      421 ggtccatctg cacgatgagc ttgtccttga agtcctgcgc gtagtagagg ttcgtgtcga
      481 cgctcaggta ctccttggcc atgtgtcggt ggtcccggta gatgagctgc gattcggcat
      541 cgttgggtat cggcggctct ggctggagct ccggatcaag aatctcggcc agtgactgtt
      601 cggtgccgtc gttctcatcc tcgtcgggat ccacgtcgac gtccaacgag ttgacaatct
      661 cctgctcatg ctcttgctcc tgcagctcct catctcttgc ttgccaacca ttcgcatggc
      721 tcagcagtcc gttcgagtgg gcgtggatgc cgccgttgtt gttggacaga tccgtggtca
      781 tcatcaccgg cttggtgtcc gtcaccgtga gtcgttcgcg gccatcgctc tttgcgttcg
      841 tgctgctgct gctgctttcg ctgggactca gatcaaagcc attcgcctcc acgtcgaggg
      901 tgagctgctc catgggcttg caggccatca ggatcatgcc gttccggatc gtttcgaggc
      961 gctgagtggc ctcagccgac gaggtgaggt tgaagctatc gttccggctc tcgttgctct
     1021 cctcctcggg aa
//
