LOCUS       sequence-108689-        1466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-MEK5-pcw107
FEATURES             Location/Qualifiers
     source          1..1466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..42
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggggtctt caaaatctaa accaaaggac cccagccagc gccggcgcag gatccgaggt
       61 taccttgact acaaagacga tgacgacaag caattgacaa gtttgtacaa aaaagttgga
      121 tgctgtggct agcccttggc ccctttcctg ccatggagaa ccaggtgctg gtaattcgca
      181 tcaagatccc aaatagtggc gcggtggact ggacagtgca ctccgggccg cagttactct
      241 tcagggatgt gctggatgtg ataggccagg ttctgcctga agcaacaact acagcatttg
      301 aatatgaaga tgaagatggt gatcgaatta cagtgagaag tgatgaggaa atgaaggcaa
      361 tgctgtcata ttattattcc acagtaatgg aacagcaagt aaatggacag ttaatagagc
      421 ctctgcagat atttccaaga gcctgcaagc ctcctgggga acggaacata catggcctga
      481 aggtgaatac tcgggccgga ccctctcaac acagcagccc agcagtctca gattcacttc
      541 caagcaatag cttaaagaag tcttctgctg aactgaaaaa aatactagcc aatggccaga
      601 tgaatgaaca agacatacga tatcgggaca ctcttggtca tggcaacgga ggcacagtct
      661 acaaagcata tcatgtcccg agtgggaaaa tattagctgt aaaggtcata ctactagata
      721 ttacactgga acttcagaag caaattatgt ctgaattgga aattctttat aagtgcgatt
      781 catcatatat cattggattt tatggagcat tttttgtaga aaacaggatt tcaatatgta
      841 cagaattcat ggatggggga tctttggatg tatataggaa aatgccagaa catgtccttg
      901 gaagaattgc agtagcagtt gttaaaggcc ttacttattt gtggagttta aagattttac
      961 atagagacgt gaagccctcc aatatgctag taaacacaag aggacaggtt aagctgtgtg
     1021 attttggagt tagcactcag ctggtgaatt ctatagccaa gacgtatgtt ggaacaaatg
     1081 cttatatggc gcctgaaagg atttcagggg agcagtatgg aattcattct gatgtctgga
     1141 gcttaggaat ctcttttatg gagcttgctc ttgggaggtt tccatatcct cagattcaga
     1201 aaaaccaggg atctttaatg cctctccagc ttctgcagtg cattgttgat gaggattcgc
     1261 ccgtccttcc agttggagag ttctcggagc catttgtaca tttcatcact cagtgtatgc
     1321 gaaaacagcc aaaagaaagg ccagcacctg aagaattgat gggccacccg ttcatcgtgc
     1381 agttcaatga tggaaatgcc gccgtggtgt ccatgtgggt gtgccgggcg ctggaggaga
     1441 ggcggagcca gcaggggccc ccgtga
//
