LOCUS       sequence-108819-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter and miR30-based hairpin module for shRNA
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK-miR-30 R4-R3
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..134)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           449..542
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           556..668
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
       61 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt
      121 tctatacaaa gttgctatcg atctagtgct gcagtattta gcatgcccca cccatctgca
      181 aggcattctg gatagtgtca aaacagccgg aaatcaagtc cgtttatctc aaactttagc
      241 attttgggaa taaatgatat ttgctatgct ggttaaatta gattttagtt aaatttcctg
      301 ctgaagctct agtacgataa gtaacttgac ctaagtgtaa agttgagatt tccttcaggt
      361 ttatatagct tgtgcgccgc ctgggtacct cggtcttcat agatctgcag cacatatact
      421 agtcgactag ggataacagg gtaattgttt gaatgaggct tcagtacttt acagaatcgt
      481 tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg
      541 ctcgagcaac cagaattcag ggggctactt taggagcaat tatcttgttt actaaaactg
      601 aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa
      661 atcacttttt tc
//
