LOCUS       sequence-108830-         398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and a non-silencing (scrambled) shRNA module. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6 n.s shRNA R4-R3
FEATURES             Location/Qualifiers
     source          1..398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(16..139)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     182..202
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     353..372
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
       61 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      121 acttttctat acaaagttgc tatcgatccg agactagcct cgagcggccg cccccttcac
      181 cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      241 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      301 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      361 tatgcttacc gtaacttgaa agtatttcga tttcttgg
//
