LOCUS       sequence-10888-e        1851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-HIPK4
FEATURES             Location/Qualifiers
     source          1..1851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1374..1388
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 atgtccacca tccagtcgga gactgactgc tacgacatca tcgaggtctt gggcaagggg
       61 accttcgggg aggtagccaa gggctggcgg cggagcacgg gcgagatggt ggccatcaag
      121 atcctcaaga atgacgccta ccgcaaccgc atcatcaaaa acgagctgaa gctgctgcac
      181 tgcatgcgag gcctagaccc tgaagaggcc cacgtcatcc gcttccttga gttcttccat
      241 gacgccctca agttctacct ggtctttgag ctgctggagc aaaacctttt cgagttccag
      301 aaggagaaca acttcgcgcc cctccccgcc cgccacatcc gtacagtcac cctgcaggtg
      361 ctcacagccc tggcccggct caaggagctg gctatcatcc acgctgatct caagcctgag
      421 aacatcatgc tggtggacca gacccgctgc cccttcaggg tcaaggtgat tgacttcgga
      481 tccgccagca ttttcagcga ggtgcgctac gtgaaggagc catacatcca gtcgcgcttc
      541 taccgggccc ctgagatcct gctggggctg cccttctgcg agaaggtgga cgtgtggtcc
      601 ctgggctgcg tcatggctga gctgcacctg ggctggcctc tctaccccgg caacaacgag
      661 tacgaccagg tgcgctacat ctgcgaaacc cagggcctgc ccaagccaca cctgttgcac
      721 gccgcctgca aggcccacca cttcttcaag cgcaaccccc accctgacgc tgccaacccc
      781 tggcagctca agtcctcggc tgactacctg gccgagacga aggtgcgccc attggagcgc
      841 cgcaagtata tgctcaagtc gttggaccag attgagacag tgaatggtgg cagtgtggcc
      901 agtcggctaa ccttccctga ccgggaggcg ctggcggagc acgccgacct caagagcatg
      961 gtggagctga tcaagcgcat gctgacctgg gagtcacacg aacgcatcag ccccagtgct
     1021 gccctgcgcc accccttcgt gtccatgcag cagctgcgca gtgcccacga gaccacccac
     1081 tactaccagc tctcgctgcg cagctaccgc ctctcgctgc aagtggaggg gaagcccccc
     1141 acgcccgtcg tggccgcaga agatgggacc ccctactact gtctggctga ggagaaggag
     1201 gctgcgggta tgggcagtgt ggccggcagc agccccttct tccgagagga gaaggcacca
     1261 ggtatgcaaa gagccatcga ccagctggat gacctgagtc tgcaggaggc tgggcatggg
     1321 ctgtggggtg agacctgcac caatgcggtc tccgacatga tggtccccct caaggcagcc
     1381 atcactggcc accatgtgcc cgactcgggc cctgagccca tcctggcctt ctacagcagc
     1441 cgcctggcag gccgccacaa ggcccgcaag ccacctgcgg gttccaagtc cgactccaac
     1501 ttcagcaacc tcattcggct gagccaggtc tcgcctgagg atgacaggcc ctgccggggc
     1561 agcagctggg aggaaggaga gcatctcggg gcctctgctg agccactggc catcctgcag
     1621 cgagatgagg atgggcccaa cattgacaac atgaccatgg aagctgagag gccagaccct
     1681 gagctcttcg accccagcag ctgtcctgga gaatggctga gtgagccaga ctgcaccctg
     1741 gagagcgtca ggggcccacg ggctcagggg ctcccacccc gccgctccca ccagcatggt
     1801 ccaccccggg gggccaccag cttcctccag catgtcaccg ggcaccactg a
//
