LOCUS       sequence-108969-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV114
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(230..235,236..253,254..260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             302..313
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
       61 gctctagaac tagtggatcc ccaaaatgag acccaatacg caaaccgcct ctccccgcgc
      121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga
      301 gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      361 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      421 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      481 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      541 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      601 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      661 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      721 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      781 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      841 cgctgaagtt aa
//
