LOCUS       sequence-109043-       12019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-frt-stop-frt-p65AD::CaM
FEATURES             Location/Qualifiers
     source          1..12019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    3502..3549
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          3669..3734
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3864..3884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4156..4290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5112..5159
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5224..5244
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5695..6066
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             6109..6552
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     intron          6648..6713
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6843..6863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7135..7269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7296..7314)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7774..7845,8240..8513,8588..9242,9304..9619,9840..9971,
                     10042..10656)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(11434..11988,11989..12019)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgagggtac ccggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat
     3541 aggaacttca gagcgctttt gaagctagag aattcgcgca ctagtgctag aggatctttg
     3601 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     3661 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     3721 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     3781 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     3841 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     3901 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     3961 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     4021 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     4081 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4141 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4261 ctcatcaatg tatcttatca tgtctggatc tgcgtcgcgc cacttcaacg ctcgatggga
     4321 gcgtcattgg tgggcggggt aaccgtcgaa atcagtgttt acgcttccaa tcgcaacaaa
     4381 aaattcactg caacactgaa aagcatacga aaacgatgaa gattgtacga gaaaccataa
     4441 agtattttat ccacaaagac acgtatagca gaaaagccaa gttaactcgg cgataagttg
     4501 tgtacacaag aataaaatcg gccagattca gtgttgtcag aaataagaaa accccactat
     4561 gtttttcttt gccttttctt tctcccagcg atcattcatt tcgtggtgaa agaacggggt
     4621 cattgcacgg agtttcgact gcgggaaagc agagctgccg ttcacttcgt ctataattag
     4681 cgctttctat tttccccgat tcgggccgct gctgcgcttt tccgcctgct gtttgtggca
     4741 agtgtagcag caggctgtgc acgcagtgtg gcatgcactt ggctttccac cgttggtatc
     4801 gattctctgg gacgatgagt cattcctttc ggggccacag cataatcgtt gccagctcac
     4861 cgaaatggtg acttcatttc ttaactgccg tcaagcatgc gattgtacat acatacatat
     4921 ttatatatgt acatatttat gtgactatgg taggtcgata taatagcaat caacgcaagc
     4981 aaatgtgtca gtcctgctta caggaacgat tctatttagt aattttcgtt gtataaagta
     5041 attatgtatg tatgtaagcc ccataaatct gaaacaatta ggcaaaacca tgcgaggatc
     5101 cgggggatct tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
     5161 gagcgctttt gaagctgggt accagatctc aacatggata aagcggaatt aattcccgag
     5221 cctccaaaaa agaagagaaa ggtcgaattg ggatccacgc cgatggagtt ccagtacctg
     5281 cccgatacgg atgaccgtca ccgtatcgaa gaaaagcgga agcgaaccta tgaaaccttc
     5341 aagtccatca tgaaaaagtc ccccttctcg ggccccacgg acccgcgccc cccgccccgt
     5401 cgtattgcgg ttccttcgcg cagcagtgcc agcgtcccca aacccgcacc gcagccctac
     5461 ccgttcactt cctcccttag cacgattaac tatgatgagt tccccacgat ggtgttcccc
     5521 agtggacaaa tttcccaggc atcggcactg gctccggccc caccgcaagt cctcccccag
     5581 gcgcccgctc cggcaccggc tcccgcaatg gtgagtgctc tggcccaggc ccccgctcca
     5641 gtccccgtgc tggcgcctgg acccccacag gcagttgccc ctcctgctcc gaaaccaacg
     5701 caggcgggcg aaggaaccct gagcgaggcc ctcttgcagc ttcagttcga tgacgaagac
     5761 ttgggagccc tgctgggtaa cagcacagac cctgccgtat tcaccgatct cgcatccgtg
     5821 gacaacagcg agtttcagca gctcttgaat cagggaatcc cggtcgcacc tcataccaca
     5881 gagcccatgc tgatggaata cccggaggct atcacgcgac tggtgaccgg cgcacagcga
     5941 ccacccgatc cagcccctgc cccactgggt gccccgggtt tgcccaatgg cctcctcagc
     6001 ggcgatgagg atttctccag catcgctgat atggatttct ccgctttgct gagccagata
     6061 agctccgcta gcggaggagg tggtggaggt ggaggtggag gtactagtga ccaactgact
     6121 gaagagcaga tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg
     6181 acaataacaa ccaaggagct ggggacggtg atgcggtctc tggggcagaa ccccacagaa
     6241 gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc
     6301 cctgagttcc tgacaatgat ggcaagaaaa atgaaagaca cagacagtga agaagaaatt
     6361 agagaagcgt tccgtgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt
     6421 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc
     6481 agggaagcag acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg
     6541 acagcgaagt aactcgaggg tacctctaga ggatctttgt gaaggaacct tacttctgtg
     6601 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     6661 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     6721 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     6781 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     6841 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     6901 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     6961 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct
     7021 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     7081 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7141 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7261 gtctggatcg gatccactag aaggccttag tatgtatgta agttaataaa accctttttt
     7321 ggagaatgta gatttaaaaa aacatatttt ttttttattt tttactgcac tggacatcat
     7381 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgaa gtaccaggtt
     7441 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
     7501 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
     7561 ccgtttgtag cgttacctag cgtcaatgtc cgccttcagt tgcactttgt cagcggtttc
     7621 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
     7681 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
     7741 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
     7801 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
     7861 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
     7921 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat cgcacaaaca aatagtgaca
     7981 cgaaacagat tattctggta gctgtgctcg ctatataaga caatttttaa gatcatatca
     8041 tgatcaagac atctaaaggc attcattttc gactacattc ttttttacaa aaaatataac
     8101 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
     8161 tcgtaggata cttcgttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
     8221 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
     8281 cttcgggcag gccaaaaact acggcacgct cctgccaccc agtccgccgg aggactccgg
     8341 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
     8401 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
     8461 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
     8521 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
     8581 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     8641 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     8701 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     8761 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     8821 ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     8881 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     8941 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     9001 gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactca
     9061 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     9121 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     9181 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     9241 tagtgagttc gatgtgttta ttaagggtat ctagcattac attacatctc aactcctatc
     9301 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     9361 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     9421 ttttgctatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatttgga
     9481 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     9541 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     9601 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     9661 ttcttggaaa caaattcgct agattttagt tagaattgcc tgattccaca cccttcttag
     9721 tttttttcaa tgagatgtat agtttatagt tttgcagaaa ataaataaat ttcatttaac
     9781 tcgcgaacat gttgaagata tgaatattaa tgagatgcga gtaacatttt aatttgcaga
     9841 tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggcgtga
     9901 tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg
     9961 ccacgataaa tgtaagtctt gtttagaata catttgcata ttaataattt actaactttc
    10021 taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc
    10081 cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattaccg
    10141 ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg
    10201 gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca
    10261 acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg
    10321 ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg
    10381 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
    10441 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
    10501 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
    10561 ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat
    10621 ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc
    10681 ttgttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
    10741 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
    10801 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
    10861 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
    10921 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
    10981 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
    11041 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag
    11101 tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct
    11161 ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact
    11221 ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag
    11281 tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt
    11341 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
    11401 tgcttggatt tcactggaac tagaattaat tcggctgctg ctctaaacga cgcatttcgt
    11461 actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat gaacaggacc
    11521 taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca aacttactct
    11581 gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg ctctatcact
    11641 ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt
    11701 gcagccttcc actgcgaatc attaaagtgg gtatcacaaa tttgggagtt ttcaccaagg
    11761 ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg cacgtgaatt
    11821 aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt tttttattcc
    11881 acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa
    11941 gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg acgggaccac
    12001 cttatgttat ttcatcatg
//
