LOCUS       sequence-109083-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUE411
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        237..312
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
       61 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
      121 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
      181 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccg gtctcggttt
      241 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      301 ccgagtcggt gctttttttt ttcgttttgc attgagtttt ctccgtcgca tgtttgcagt
      361 tttattttcc gttttgcatt gaaatttctc cgtctcatgt ttgcagcgtg ttcaaaaagt
      421 acgcagctgt atttcactta tttacggcgc cacattttca tgccgtttgt gccaactatc
      481 ccgagctagt gaatacagct tggcttcaca caacactggt gacccgctga cctgctcgta
      541 cctcgtaccg tcgtacggca cagcatttgg aattaaaggg tgtgatcgat actgcttgct
      601 gctaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg
      661 agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga
      721 agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct
      781 atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt
      841 ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca
      901 tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc cattttatta
      961 gtacatccat ttagggttta gggttaatgg
//
