LOCUS       sequence-109150-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An empty optimized gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtttaaactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
       61 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
      121 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
      181 gagagactct ggtctaacca gagagaccca gtacaggcaa aaagcagatc ttgacttctt
      241 tgggagtgaa ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca
      301 gctgccttgt aagtcattgg tcttaaaggt acctagcggc cgctcaatta agcttgtgcc
      361 ccagtttgct agggaggtcg cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg
      421 tctcgttgtt ggcctccttg attctttcca gtctgtagtc cacatagtag acgccaggca
      481 tcttgaggtt cttagcgggt ttcttggatc tatatgtggt cttgatgttt gcgatcagat
      541 ggctcccgcc cacgagcttc agggccatgt cgtttctgcc ttccaggccg ccgtcagcgg
      601 ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt cttctgcatc acagggccgt
      661 tggatgtgaa gttcacccct ctgatcttga cgttgtagat gaggcagccg tcctggaggc
      721 tggtgtcctg ggtagcggtc agcacgcccc cgtcctcgta tgtggtgact ctctcccatg
      781 tgaagccctc agggaaggac tgcttgaaga agtcggggat gccctgggtg tggttgatga
      841 aggtcttgct gccgtagagg aagctagtag ccaggatgtc gaaggcgaag gggagagggc
      901 cgccctcgac caccttgatt
//
