LOCUS       sequence-109332-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Expression of genomic anchor 
            probe, containing dCas9 and CIB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: NLS-dCas9-trCIB1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta ccggtgccac catgtaccca tacgatgttc
      121 cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag aagtacagca
      181 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
      241 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
      301 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
      361 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
      421 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
      481 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
      541 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
      601 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
      661 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
      721 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
      781 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
//
