LOCUS       sequence-109377-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; 
            drosophila vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAWG-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..584)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 agaagctggg tttcaaacag agatgtgtcg aagatggaca gtcctgtgct gatatgcatg
       61 gcatgcaaga ggcactcgtc gttcaggaag gtatccagaa tctcgttcaa ttccggggtc
      121 aggggtgagt ccaggttcag atcctcggtc atggactcaa gtgtggttgt cagctcatcc
      181 agatggcccc ttgggggcgg atgggaaagg tccatttggc cacagattgc agcctcttcc
      241 ttctggggaa tcacagtatc ggccatctcc cgaagggctt tgacagcctg gctcgtctct
      301 tcatcgggat cctccaacag gtgactggcc tcgggagtca ctgcgggcgc tggatccagt
      361 ggctgaggga ctggtgccgg ggtcagtgac ccgactggct catgtactgg accggttggt
      421 gttggtgcga ggctggcggg gagtgggcgg ttggcccatg gacttcctgg aggatgaaat
      481 ggccggattc gttttggctg gcacacctcg cggccctcaa acacgtcact aatagcggag
      541 ccggcctcag gcttcggcaa aaacatccct tccctggaat cccgagatcc cgatccgcta
      601 cccagcagtg cggagaagtc catgtcggcg atagagctga agtcctcgtc gccagacagc
      661 agtccattag gcaggcctgg tgctcccaga ggggcaggag ctggatcagg aggcctctga
      721 gcgcctgtca cgagccgggt gatggcctcg gggtattcca tcagcatggg ctcggtggtg
      781 tgaggggcca cagggatgcc ctggttcagc agctgctgga actcgctgtt
//
