LOCUS       sequence-109427-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Lentiviral expression of 
            IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: sgRNA4_IL1RN
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        57..132
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        257..560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        561..764
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     715..735
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(798..817)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gagactataa gtatcccttg gagaaccacc ttgttgggag tcaccctcct ggaaacgttt
       61 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      121 ccgagtcggt gctttttttc tcgagtacta ggatccatta ggcggccgcg tggataaccg
      181 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      241 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      301 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      361 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      421 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      481 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      541 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      601 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      661 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      721 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      781 agatccgcta gcccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
      841 acgtccc
//
