LOCUS       sequence-109550-        2390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-4
FEATURES             Location/Qualifiers
     source          1..2390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             777..785
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             789..1466
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MKASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     RBS             1480..1488
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2285..2371
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagaagaa aggctcagat aagcttacga gaggaaacaa tggtcgacaa gggcgaagaa
       61 ctctttaccg gcgtcgtccc gatcctggta gagcttgacg gcgatgtcaa cggtcataag
      121 tttagcgtta gcggcgaagg cgagggcgac gcgacgtacg gtaaattaac cttaaaattc
      181 atctgtacaa ccggtaaatt accggtaccg tggcccacgt tagttaccac ccttacctgg
      241 ggcgtccagt gcttcgcgcg ctatccagac cacatgaagc aacacgattt cttcaaatcg
      301 gctatgcccg aaggctatgt acaggagcgc accatcttct tcaaggacga cggtaattat
      361 aaaacccgcg cagaagttaa gttcgagggc gatacgctcg ttaatcgcat cgaactcaaa
      421 ggtatcgact tcaaagagga cggcaacatt ttaggtcaca agttagagta taacgcgatt
      481 tccgataacg tctacattac cgccgataaa caaaagaacg gtatcaaagc aaactttaag
      541 attcgtcata acatcgagga cggctcggta cagctcgccg accattacca acagaatacg
      601 ccgatcggcg acggtccggt cctcctcccg gacaatcatt atctcagcac ccagagcgcg
      661 ctctccaaag atccaaacga gaagcgcgac cacatggtcc ttctcgaatt tgtaacggcg
      721 gcaggcatca ccctcggtat ggacgaactc tataaggacg tcaaaaaata cctccgtaag
      781 gaggtatcat gaaagcgagc tccgaagacg ttatcaaaga atttatgcgt tttaaagttc
      841 gcatggaggg ctcggttaac ggtcacgagt tcgaaatcga aggggaaggc gaaggtcgtc
      901 cgtacgaagg tacccagacc gctaaactca aagttaccaa aggcggtccc ttaccgttcg
      961 cttgggatat tttaagcccg cagttccagt acggttccaa agcttacgtt aaacacccgg
     1021 cggacatccc ggactacctt aaattatcct tccccgaagg gtttaagtgg gagcgcgtta
     1081 tgaacttcga ggacggcggc gtcgttaccg ttacccagga ctcctccctt caagacggcg
     1141 agttcatcta caaagttaaa cttcgcggta ccaacttccc atccgatggt ccggtcatgc
     1201 agaagaagac catgggttgg gaagcttcca ccgagcgcat gtatccggag gacggcgctt
     1261 taaaaggcga aatcaagatg cgcttaaagt taaaagacgg cggtcactac gacgcagaag
     1321 ttaaaacgac gtacatggcc aagaaaccgg ttcagttacc gggcgcttac aaaaccgaca
     1381 tcaaactcga catcaccagc cacaacgaag actacaccat cgtcgaacag tacgaacgcg
     1441 cagaaggtcg tcacagcacc ggtgctgaat tcgtacacct aaggaggtgt acagcatgaa
     1501 acgtaaactc gaggaacttt tcacgggggt cgtcccaatt ctggtagaat tagacggtga
     1561 tgtaaacggg cacaagttta gcgtcagcgg agagggcgag ggggacgcca cctacggtaa
     1621 acttaccctt aagtttattt gcacgacggg caaattaccc gttccgtggc caacgctcgt
     1681 cactactttc ggctacggcg ttcagtgctt cgcgagatat ccggatcaca tgaagcagca
     1741 cgatttcttc aagtcggcca tgccagaggg ttacgttcag gagagaacta tatttttcaa
     1801 ggacgacggc aattacaaga cacgcgccga agtcaagttc gaaggcgata ccctggttaa
     1861 ccgtatcgag ttaaaaggta tcgattttaa agaagacgga aacattctcg gtcacaaact
     1921 cgaatacaac tataacagcc ataatgtcta cattatggct gacaaacaga agaacggcat
     1981 taaggttaac ttcaaaattc gccacaacat cgaagacggt agcgtccaac tagcagacca
     2041 ttatcaacaa aatactccta tcggcgacgg cccagtcctt ttaccagaca accattacct
     2101 tagcacacaa agcgccctta gcaaagatcc caacgagaag cgcgaccaca tggtccttct
     2161 cgaatttgtc acagcggcag ggatcaccca cggcatggac gaactctaca aataataatt
     2221 aattaaaaaa aaaaaccccg cccctgacag ggcggggttt ttttttctaa tatgcggccg
     2281 ccatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2341 gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagagcatgc
//
