LOCUS       sequence-109553-        2389 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-8
FEATURES             Location/Qualifiers
     source          1..2389
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             776..784
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             788..1465
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MKASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     RBS             1479..1487
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2284..2370
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagaagat ttacacacaa ccatcgagga ggacaacaat ggtcgacaag ggcgaagaac
       61 tctttaccgg cgtcgtcccg atcctggtag agcttgacgg cgatgtcaac ggtcataagt
      121 ttagcgttag cggcgaaggc gagggcgacg cgacgtacgg taaattaacc ttaaaattca
      181 tctgtacaac cggtaaatta ccggtaccgt ggcccacgtt agttaccacc cttacctggg
      241 gcgtccagtg cttcgcgcgc tatccagacc acatgaagca acacgatttc ttcaaatcgg
      301 ctatgcccga aggctatgta caggagcgca ccatcttctt caaggacgac ggtaattata
      361 aaacccgcgc agaagttaag ttcgagggcg atacgctcgt taatcgcatc gaactcaaag
      421 gtatcgactt caaagaggac ggcaacattt taggtcacaa gttagagtat aacgcgattt
      481 ccgataacgt ctacattacc gccgataaac aaaagaacgg tatcaaagca aactttaaga
      541 ttcgtcataa catcgaggac ggctcggtac agctcgccga ccattaccaa cagaatacgc
      601 cgatcggcga cggtccggtc ctcctcccgg acaatcatta tctcagcacc cagagcgcgc
      661 tctccaaaga tccaaacgag aagcgcgacc acatggtcct tctcgaattt gtaacggcgg
      721 caggcatcac cctcggtatg gacgaactct ataaggacgt caaaaaatac ctccgtaagg
      781 aggtatcatg aaagcgagct ccgaagacgt tatcaaagaa tttatgcgtt ttaaagttcg
      841 catggagggc tcggttaacg gtcacgagtt cgaaatcgaa ggggaaggcg aaggtcgtcc
      901 gtacgaaggt acccagaccg ctaaactcaa agttaccaaa ggcggtccct taccgttcgc
      961 ttgggatatt ttaagcccgc agttccagta cggttccaaa gcttacgtta aacacccggc
     1021 ggacatcccg gactacctta aattatcctt ccccgaaggg tttaagtggg agcgcgttat
     1081 gaacttcgag gacggcggcg tcgttaccgt tacccaggac tcctcccttc aagacggcga
     1141 gttcatctac aaagttaaac ttcgcggtac caacttccca tccgatggtc cggtcatgca
     1201 gaagaagacc atgggttggg aagcttccac cgagcgcatg tatccggagg acggcgcttt
     1261 aaaaggcgaa atcaagatgc gcttaaagtt aaaagacggc ggtcactacg acgcagaagt
     1321 taaaacgacg tacatggcca agaaaccggt tcagttaccg ggcgcttaca aaaccgacat
     1381 caaactcgac atcaccagcc acaacgaaga ctacaccatc gtcgaacagt acgaacgcgc
     1441 agaaggtcgt cacagcaccg gtgctgaatt cgtacaccta aggaggtgta cagcatgaaa
     1501 cgtaaactcg aggaactttt cacgggggtc gtcccaattc tggtagaatt agacggtgat
     1561 gtaaacgggc acaagtttag cgtcagcgga gagggcgagg gggacgccac ctacggtaaa
     1621 cttaccctta agtttatttg cacgacgggc aaattacccg ttccgtggcc aacgctcgtc
     1681 actactttcg gctacggcgt tcagtgcttc gcgagatatc cggatcacat gaagcagcac
     1741 gatttcttca agtcggccat gccagagggt tacgttcagg agagaactat atttttcaag
     1801 gacgacggca attacaagac acgcgccgaa gtcaagttcg aaggcgatac cctggttaac
     1861 cgtatcgagt taaaaggtat cgattttaaa gaagacggaa acattctcgg tcacaaactc
     1921 gaatacaact ataacagcca taatgtctac attatggctg acaaacagaa gaacggcatt
     1981 aaggttaact tcaaaattcg ccacaacatc gaagacggta gcgtccaact agcagaccat
     2041 tatcaacaaa atactcctat cggcgacggc ccagtccttt taccagacaa ccattacctt
     2101 agcacacaaa gcgcccttag caaagatccc aacgagaagc gcgaccacat ggtccttctc
     2161 gaatttgtca cagcggcagg gatcacccac ggcatggacg aactctacaa ataataatta
     2221 attaaaaaaa aaaaccccgc ccctgacagg gcggggtttt tttttctaat atgcggccgc
     2281 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2341 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agagcatgc
//
