LOCUS       sequence-109584-         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses crtEBI genes for neurosporene production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: pBad_crtEBI_14
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      138..209
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      225..252
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
       61 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
      121 gaacctaggc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      181 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      241 ggcctttctg cgtttatacc acagcacagc catatggcat tcacaaccgc gaaaaaaaac
      301 cccgccctgt caggggcggg gttttttttt acacagcaga cgtcgaagat ctttattaag
      361 caccggtgct gtgacgacct tgtgtgtgtg tgtgttatgg ttattctgct gccaggcgct
      421 cagccggttc agcttcacgg cgcgcacgcg gcgcatccgg agccagtttc gccatgacct
      481 ccgcagagcc aataacaccc ggtacaccgg cacctgggtg ggtgcccgca ccaaccagaa
      541 acagatttgc gacttcttca gagatattat gcggacggaa ccaagcagat tg
//
