LOCUS       sequence-109604-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N4-R5-YRNRV RNAP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Meyer AJ, Ellefson JW, Ellington AD
  TITLE     Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase 
            Variants for in Vivo or in Vitro Synthetic Circuitry.
  JOURNAL   ACS Synth Biol. 2014 Oct 7.
  PUBMED    25279711
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Oct 7."
COMMENT     Sequence Label: pQE-WT-N4-R5-YRNRV
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctggatctat caacaggagt ccaagctcag ctaattaagc ttttacgcga acgcgaagtc
       61 cgactctaag atgtcacgga ggttcaagtt acctttagcc ggaagtgctg gcattttgtc
      121 caattgagac tcgtgcaact ggtcagcgaa ctggtcgtag aaatcagcca gtacatcaca
      181 agactcatat gtgtcaacca tagtttcgcg cactgctttg aacaggttcg cagcgtcagc
      241 cggaatggta ccgaaggagt cgtgaatcag tgcaaaagat tcgattccgt acttctcgtg
      301 tgcccacact acagtcttac gaaggtggct accgtcttgg ctgtgtacaa agttaggagc
      361 gataccagac acctgtttgc gtgcattaat ctcgctatct ttgttggtgt taatggtcag
      421 cgccatgcga cgggtaccga ggatcacgca gtcaatgcgc gtctgaatag gcttcttgta
      481 ttcctgccac acagggaaac catcaggagt tacccaatgc acagcgcaac gcttgcgaag
      541 aatctctcca gtcttcttat ctttgacctc agcagccagc agcttagcag cagacttaag
      601 ccagttcatt gcttcaaccg cagctaccac cgtcacgctc acagattccc aaatcagctt
      661 agccatgtat ccagcagcct gatacggctg agtgaacatc agacccttgc cggaatcaat
      721 agctggctga atggtatctt ccagcacttg ttgacggaag ccgaactctt tggacccgta
      781 agccagcgtc atgactgaac gcttagtcac actgcgagta acaccgtaag ccagccattg
      841 accagccagt gccttagtgc ccagcttgac tttctcag
//
