LOCUS       sequence-109609-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  K1F-R5-IRH RNAP mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Meyer AJ, Ellefson JW, Ellington AD
  TITLE     Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase 
            Variants for in Vivo or in Vitro Synthetic Circuitry.
  JOURNAL   ACS Synth Biol. 2014 Oct 7.
  PUBMED    25279711
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Oct 7."
COMMENT     Sequence Label: pQE-WT-KIF-R5-IRH
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatctatcaa caggagtcca agctcagcta attaagcttt tacgcgaacg cgaagtccga
       61 ctctaagatg tcacggaggt tcaagttacc tttagccgga agtgctggca ttttgtccaa
      121 ttgagactcg tgcaactggt cagcgaactg gtcgtagaaa tcagccagta catcacaaga
      181 ctcatatgtg tcaaccatag tttcgcgcac tgctttgaac aggttcgcag cgtcagccgg
      241 aatggtaccg aaggagtcgt gaatcagtgc aaaagattcg attccgtact tctcgtgtgc
      301 ccacactaca gtcttacgaa ggtggctacc gtcatggctg tgtacaaagt taggagcgat
      361 accagactcc tgtttgcgtg catcaatctc gctatctttg ttggtgttaa cggtaggctg
      421 taagttgaaa ctaccgagga acattatgtt caagcgcgtc tgaataggct tcttgtattc
      481 ctgccacaca gggaaaccat caggagttac ccaatgcaca gcgcaacgct tgcgaagaat
      541 ctctccagtc ttcttatctt tgacctcagc agccagcagc ttagcagcag acttaagcca
      601 gttcattgct tcaaccgcag ctaccaccgt cacgctcaca gattcccaaa tcagcttagc
      661 catgtatcca gcagcctgat tcggctgagt gaacatcaga cccttgccgg aatcaatagc
      721 tggctgaatg gtatcttcca gcacttgttg acggaagccg aactctttgg acccgtaagc
      781 cagcgtcatg actgaacgct tagtcacact gcgagtaaca ccgtaagcca gccattgacc
      841 agccagtgcc ttagtgccca gcttgacttt ctcagagatt tcaccagtgt tctcatcg
//
