LOCUS       sequence-109710-        3539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MXS_chaining vector with CMV::tTA2-bGHpA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_CMV::tTA2-bGHpA
FEATURES             Location/Qualifiers
     source          1..3539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(647..1264,1265..1270,1271..1306,1307..1309,1310..1345,
                     1346..1348,1349..1384,1385..1393)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    1400..1624
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1647..1751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1752..1820,1821..2612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1970..1989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2783..3371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3272..3291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatatca aacgcgtaac tcgagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatccgctag ggatctcagc tggtcgagtc gccaccatgt ctagactgga
      661 caagagcaaa gtcataaact ctgctctgga attactcaat gaagtcggta tcgaaggcct
      721 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
      781 gaagaacaag cgggccctgc tcgatgccct ggcaatcgag atgctggaca ggcatcatac
      841 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
      901 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
      961 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     1021 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     1081 attggaggat caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     1141 tatgccccca cttctgagac aagcaattga gctgttcgac catcagggag ccgaacctgc
     1201 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     1261 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     1321 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     1381 gctccccgga tgagtcgagc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     1441 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     1501 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     1561 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     1621 atggtcgacc aattcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1681 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1741 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1801 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1861 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1921 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1981 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2041 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2101 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2161 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2221 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2281 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2341 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2401 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2461 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2521 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2581 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2641 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2701 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2761 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2821 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2881 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2941 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3001 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3061 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3121 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3181 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3241 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3301 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3361 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3421 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3481 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagc
//
